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RPMI 1640 Medium, HEPES 22400105

产品介绍【返回】

品牌: Gibco
货号: 22400105
规格: 500ml

描述

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. We offer a variety of RPMI 1640 Medium modifications for a range of cell culture applications. Find the right formulation using the .
This RPMI is modified as follows:
With     Without
• L-glutamine
• HEPES
• Phenol Red  

The complete  is available.
Using RPMI
RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10%  (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Product intended use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco® RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II  (DMF).
cGMP manufacturing and quality system
RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical RPMI 1640 product made in our Scotland facility (52400-041). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
 

规格

Glutamine: L-Glutamine
Phenol Red Indicator: Phenol Red
Form: Liquid
HEPES Buffer: HEPES
Serum Supplementation: Standard Serum Supplementation
Sodium Pyruvate Additive: No Sodium Pyruvate
Product Line: Gibco®
Product Size: 10 x 500 mL
Volume (Metric): 500 ml
Green Features: Sustainable packaging
Shipping Condition: Room Temperature
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