黄金棋牌

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试剂耗材

Polyethylenimine Linear(PEI)线性化聚乙烯亚胺(MW25000)

产品介绍【返回】

货号: FSF0001-1G
供应商: 上海觅拓
规格: FSF0001-1G/FSF0001-2G
规格: FSF0001-1G 产品价格: ¥1450.0
规格: FSF0001-2G 产品价格: 询价
简单介绍:
Polyethylenimine Linear(PEI)线性化聚乙烯亚胺(MW25000)聚乙烯亚胺(Polyethylenimine)Max—高效线性PEI:可用作转染试剂,也可用于抗菌剂包被。Polyethylenimine "Max"与普通的线性化Polyethylenimine多聚物骨架相同。高效线性PEI含有更多的自由基(质子数),比普通PEI高11%。重复的乙烯亚胺单元平均数量约为581,因此,显著提高了质子化氮的长度。CAS号:9002-98-6 分子式:(C2H5N)x Polyethylenimine Linear(PEI)线性化聚乙烯亚胺(MW25000)
详情介绍:

Polyethylenimine Linear(PEI)线性化聚乙烯亚胺(MW25000)

产品简介:

中文名称:黄金棋牌PEI转染试剂 ,线性聚乙烯亚胺

英文名称:Polyethylenimine Linear(PEI)

FSF0001-1G Polyethylenimine Linear(PEI)线性化聚乙烯亚胺(MW25000) 1g 1450.00  进口分装
FSF0001-2G Polyethylenimine Linear(PEI)线性化聚乙烯亚胺(MW25000) 2g 询价 进口分装
 

产品应用:

      XIANXINGHUAJUYIXIYAANPEI 25,000,YIZHONGGAODIANHEYANGLIZIJUHEWU,FEICHANGRONGYIJIEHEYUGAODIANHEYINLIZIJIZHI。GONGYEYINGYONGSHANG,XIANXINGHUAPEIKEGAISHANFUDIANHERANLIAODEWULIWAIGUAN,YIDIAOZHENGRANLIAODEHUAXUETEXINGHEZENGQIANGRANLIAOYUGUXIANGJIZHIDENIANFUNENGLI,CHANGYONGZUONIANFUZENGQIANGJI,ZUOYONGTONGDUOJULAIANSUAN(poly-lysine);KEYANYINGYONGSHANG,XIANXINGHUAPEINENGYUDNAHUOQITAFUDIANHESHENGWUDAFENZIJIANDANJIEHE,ZHENGSHIZHEIYITEXING,SHIDECHENGWEIYIZHONGFEICHANGXINGZHIYOUXIAODEZAITIYUNSHUJIEZHI(Vector carrier),JIZHUANRANSHIJI。XIANXINGPEIZHUANRANSHIJISHIYIZHONGYANGLIZIJUHEWU,FENZILIANG25000,TANENGYUHESUANXINGCHENGFUHEWU,BINGSHIGAIFUHE WUJINRUBURUDONGWUXIBAO。XIANXINGPEIZHUANRANSHIJIGUANGFANSHIYONGYUCHANGJIANXIBAOXI,RU HEK-293、HEK293T、 Hep G2、Hela、CHO-K1、COS-1、COS-7、NIH/3T3 HE Sf9 DENG。GAISHIJIJISHIZAIYOUXUEQINGCUNZAIDEQINGKUANGXIA, TARENGRANNENGGAOXIAODEJIANGHESUANDAORUXIBAO。XIANXINGPEIZHUANRANSHIJIJUYOUYOUYUEDEZHUANRANXIAOLV , ZHONGZUDANBAIDEGAOBIAODASHUIPING ,YUHANXUEQINGDEPEIYANGJIXIANGJIANRONG, DIXIBAODUXING,YIYUCAOZUO !

Polyethylenimine “Max”, (nominally Mw 40,000*) - High Potency Linear PEI (Equivalent to Mw 25,000 in Free Base Form)GAOZHUANHUAXIAOLVDEPEI Max 40,000,ZIYOUJIXINGSHIFENZILIANGXIANGDANGYUPEI 25,000,YANJIUZHENGSHIQITIWAIZHILIDNAZHUANRANXIAOLVTIGAODA21BEI。

产品属性:

CAS:9002-98-6, 26913-06-4

分子式:(CH2CH2NH)n

熔点:73-75℃

溶解性:黄金棋牌溶于热水、低pH冷水, 甲醇和乙醇。不溶于: Et2O、******和冷水。

外观:白色到黄色固体

储存:RT室温

使用方法【来源于:CSH protocols,仅作交流学习】 :

1. Pour ~450 mL of Milli-Q H2O into a 500-mL glass beaker.

2. Add 500 mg of PEI to the beaker with stirring.

黄金棋牌 3. Add concentrated HCl drop-wise to the solution to pH <2.0.

黄金棋牌 4. Stir until the PEI is dissolved (~2-3 h). Maintain the pH <2.0 throughout.

黄金棋牌 Approximately 800 μL of 12 M HCl will be required for full PEI dissolution. There may still be some small fiber particles that will not dissolve.

5. Add concentrated NaOH drop-wise to the solution to pH 7.0.

Approximately 500 μL of 10 M NaOH will be required to neutralize the PEI solution.

6. Pour the solution into a 500-mL glass cylinder. Adjust the final volume to 500 mL with Milli-Q H2O.

黄金棋牌 7. Filter-sterilize the solution through a 0.22-μm membrane.

黄金棋牌 8. Store aliquots of the desired volume at -20°C.

ZHUYISHIXIANG

1)WEILENINDEANQUANHEJIANKANG,QINGCHUANSHIYANFUBINGDAIYICIXINGSHOUTAOCAOZUO。

Reference(s)参考文献:

黄金棋牌 Siegman S, Truong NF, Segura T. (2015). Encapsulation of PEGylated low-molecular-weight PEI polyplexes in hyaluronic acid hydrogels reduces aggregation. Acta Biomater. in Press, doi:10.1016/j.actbio.2015.09.020

黄金棋牌 Baranyi, L. et al. Rapid Generation of Stable Cell Lines Expressing High Levels of Erythropoietin, Factor VIII, and an Antihuman CD20 Antibody Using Lentiviral Vectors. Human Gene Therapy Methods 24, 214–227 (2013). doi:10.1089/hgtb.2013.002

黄金棋牌 Wulhfard, S., Baldi, L., Hacker, D. L. & Wurm, F. Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells. Journal of Biotechnology 148, 128–132 (2010). doi:10.1016/j.jbiotec.2010.05.003

黄金棋牌 Thomas M, Lu JJ, Ge Q, Zhang C, Chen J, Klibanov AM. (2005). Full deacylation of polyethylenimine dramatically boosts its gene delivery efficiency and specificity to mouse lung. Proc Natl Acad Sci U S A.102(16):5679-84.

黄金棋牌 Durocher, Y., Perret, S. & Kamen, A. High-level and high-throughput recombinant protein production by transient transfection of suspension-growing human 293-EBNA1 cells. Nucleic acids research 30, E9 (2002). doi:10.1093/nar/30.2.e9

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